The Keene Springs Hotel is a rambling wood-frame, two-story Greek Revival-style building built in sections in 1841 by Mason Singleton Jr. in the hamlet of Keene, near Nicholasville, Kentucky in Jessamine County. He and his wife Nancy owned and operated the hotel and tavern as a resort destination for the white sulphur springs nearby. During the cholera epidemic of 1848–1849 and outbreaks in the early 1850s, residents of Lexington came to the hotel to try to escape the spread of disease in the city. The Singletons operated the hotel until 1857, when they sold it to Alfred McTyre.